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KMID : 0390119930330010265
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Journal of Pusan Medical College
1993 Volume.33 No. 1 p.265 ~ p.274
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Detection of M. leprae by PCR
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Abstract
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The polymerase chain reaction(PCR), using specific oligonucleotide primers and Taq DNA polymerase, has provided a method to detect pathogens of several infectious diseases. PCR based on the selective amplification of a 372 bp fragment of the
genome
encoding species-specific repetitive sequence of Mycobacterium leprae was applied on sections of 36 paraffin-embedded biopsy samples from the various types of untreated leprosy patients.
@ES The results were as follows:
@EN 1. Amplification of the Mycobacterium leprae DNA in samples from all multibacillary and paucibacillary leprosy patients was detected.
2. A failed PCR could be recovered by using dilution of the source DNA, presumably because inhibitors were also diluted out.
3. Amplifications of the Mycobacterium leprae DNA in samples from tuberculosis and Mycobacterium marinum infected patients were not detected.
Therefore, PCR has shown here to be a useful tool for the detection of Mycobacterium leprae in paraffin-embedded biopsy samples from the various types, especially paucibacillary type of leprosy patients.
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